Sonicated herring sperm dna
WebThe chromosome-specific repetitive DNA probes were denatured for 10 min at 72C in a hybridization mixture containing 2 μg/ml probe DNA, 500 μg/ml sonicated herring sperm DNA (Sigma), 0.1% Tween-20, 10% dextran sulfate, and 60% formamide in 2 × SSC at pH 7.0. WebApr 6, 2016 · A 25 µL of fixed cells were added to 150 μL of staining buffer (PBS, 1 g L −1 BSA, 0.1 g L −1 RNaseA, phosphatase inhibitors (10 mM NaF, 1 mM Na 2 MoO 4, 1 mM NaVO 3), 0.25 g L −1 sonicated herring sperm DNA, 0.1% Triton X-100, and 5 mM EDTA), supplemented by mouse monoclonal anti-H2AXS139P-FITC conjugated antibody (Merck …
Sonicated herring sperm dna
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WebDeoxyribonucleic acid from herring sperm. single-stranded for hybridization. View Price and Availability. Sigma-Aldrich. D7656. Deoxyribonucleic acid, single stranded from salmon … WebSigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques. …
WebCheck molecular size of DNA by gel elec-trophoresis; it should be 200–400 bp. Store in 50-µl aliquots for up to 1 year at –20°C. DNA, Sonicated Herring Sperm (2 mg/ml) Resuspend 1 … WebApr 26, 2024 · Approximately 10 μg/ml of sonicated herring sperm DNA (Sigma-Aldrich) was coated on ELISA plates. The antibodies alkaline phosphatase-conjugated anti-IgM and anti-IgG (SouthernBiotech) were used. Statistical analyses. The experimental data were represented as the mean ± standard deviations (SD).
WebHerring Sperm DNA. DNA Type: Sperm DNA; Species: Fish; Tissue: Testis; Citations: Supplier Page. Compare Product + See More (1) View All (3) Abnova Corporation Salmon Sperm DNA, Sonicated. DNA Type: Sperm DNA; Species: Fish, Salmon; Tissue: Testis; Supplier Page. Compare Product. Select All. Select up to 5 products from above to compare or ... Webtained sonicated herring sperm DNA at 250-500 ,/g/ml. Hy-bridization buffer was 35-50% (vol/vol) in deionized form-amide and contained 250-500 ,g of sonicated DNA per ml. Filters were subjected to prehybridization treatment for 2-4 hr at 42?C and were hybridized at 42?C under probe concentration and time conditions given in Results.
WebNov 26, 2024 · Key proteins transferred by epididymal extracellular vesicles (EVs) to the transiting sperm cells contribute to their centrosomal maturation and developmental potential. Although not reported in sperm cells yet, galectin-3-binding protein (LGALS3BP) is known to regulate centrosomal functions in somatic cells. Using the domestic cat model, …
Webprobe DNA, 500 m g/ml sonicated herring sperm DNA (Sigma), 0.1% Tween-20, 10% dextran sulfate, and 60% formamide in 2 3 SSC at pH 7.0. The slides were then incu-bated overnight at 37C in a moist chamber and subsequently washed. Histochemical detection of the biotinylated DNA probes was performed by the standard avidin–biotin com- highwater filters couponWebSDS, 0.1 mg/ml sonicated herring sperm DNA] (Sambrook et al., 1989; Yoon et al., 1989; Tamkun et al., 199 1). Southern blots were washed as described (Stoffers et al., 1989) except that the higher-stringency wash was at 37°C instead of 50°C. Phages showing strong hybridization were highwater festival 2018WebThe aim of this study was to develop casein-based nanoscale carriers as a potential delivery system for daunorubicin, as a pH-responsive targeting tool for acute lymphocytic leukemia. A coacervation technique followed by nano spray-drying was used highwater fest ticketsWebDec 20, 2007 · Protein A-sepharose beads (∼5 mg) pre-blocked with 10 μg sonicated herring sperm DNA and BSA (0.02% final concentration after addition to chromatin) were then added and samples rotated for a further 2 h at 4°C. Beads were recovered by centrifugation, and unbound supernatant chromatin was retained. highwater farm silt coWebSonicated herring sperm DNA at a concentration of 1 mM (bp) in BPE buffer was used for all experiments. A scan rate of 1°C min –1 was used. Primary data were corrected by … highwater fish farm huntsburgWebThe DNA is mixed with the beads, which are then suspended in an oil emulsion, prior to amplification. Typically, mixing three molecules of DNA per bead provides a suitable level … highwater fest 2022Webhybridized in 1 M NaCl, 0.1% SDS, 1.5 mg/mL herring sperm DNA, and 10% dextran for 3 hours at 65°C and then hybridized with the appropriate radiolabeled probe at 65°C for 12-24 hours in the prehybridization solution with the addition of 1.5 mg/mL sonicated herring sperm DNA. Blots were washed to various stringencies, depend- small sized washing machine