Phospho-mtor antibody
WebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein. WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
Phospho-mtor antibody
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WebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies … WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with 150 nM insulin (Ins) for 6 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 ...
WebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide … WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly …
WebProduct name Anti-mTOR (phospho S2481) antibody [EPR427 (N)] See all mTOR primary antibodies Description Rabbit monoclonal [EPR427 (N)] to mTOR (phospho S2481) Host species Rabbit Tested applications Suitable for: WB, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP Species reactivity Reacts with: Human WebImmunofluorescent analysis of Phospho-mTOR (Ser2481) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X …
WebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies Cells (fibroblast, MRC-5 human cell lines) were starved form 4 hours (0,5% FBS) and induced with TGF-b. eIF4E siRNA was transfected with lipofectamine RNAmax for 24 hours.
WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor … ray albright steelWeb80596-1-RR targets Phospho-mTOR (Ser2448) in WB, ELISA applications and shows reactivity with Human, Rat samples. Background Information MTOR, also named as … ray albright okcWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold ray alburty company joplin moWebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. rayal current rate in pakWebThe Phospho-mTOR (Ser2481) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2481) Antibody has been validated for the following applications: Western Blot. rayaldee copay assistanceWebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. rayaldee assistanceWebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-AKT/ GSK3 beta / mTor and total-AKT/ GSK3 beta / mTor. For each target, a capture antibody has been coated onto microwells. ray-al coaches limited